New Version of IMAC Based Purification Reagent

PhosPRO™ provides a new version of IMAC based purification method and delivers excellent purification result. PhosPRO™ can specifically purify the phosphoprotein from the protein sample by simple process with our proprietary chromatographic technology. PhosPRO™ can also successfully purify phosphoprotein from different biological materials, such as animal, plant, and microorganism, and is suitable for applying quality required analysis.

Special Characteristic

More flexible:PhosPRO™ provides stringent buffer stock for customer to optimize washing condition for their sample and to maximize target phosphoprotein purification result.
 

More choices:PhosPRO™ series have 2 versions with 2 different packages for different purposes

   a. Pre-packed column package is designed for proteomics research and can purify phosphoprotein within 1 hour. The purified sample can directly be analyzed by two-dimensional electrophoresis or mass spectrometry.

   b. Evaluation package is designed for phosphoproteomics research and researcher can small scale optimizes suitable chromatographic conditions for the target phosphoprotein.

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Product Detail :

Figure 1. Purification efficacy of PhosPRO™ Phosphoprotein Purification Kit

 (A) and Brand Q kit (B). Protein standard markers contain glycogen phosphorylase b (GP), bovine serum albumin (BSA), ovalbumin (OVA), carbonic anhydrase (CA), trypsin inhibitor (TI), and lactalbumin (LAC) were used for evaluation. Ovalbumin was the only phosphoprotein in the test material. F: flow through; E: eluent; VisPRO™ 5 minute Protein Stain Kit (VisPRO™) and Pro-Q Diamond stain (Pro-Q, from Invitrogen) were used for developing total proteins or phosphoproteins.

Figure 2. Optimize suitable chromatographic condition with PhosPRO™ Phosphoprotein Purification Kit.

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Product Detail :

 

 

Figure 1. Purification efficacy of PhosPRO™ Phosphoprotein Purification Kit

 (A) and Brand Q kit (B). Protein standard markers contain glycogen phosphorylase b (GP), bovine serum albumin (BSA), ovalbumin (OVA), carbonic anhydrase (CA), trypsin inhibitor (TI), and lactalbumin (LAC) were used for evaluation. Ovalbumin was the only phosphoprotein in the test material. F: flow through; E: eluent; VisPRO™ 5 minute Protein Stain Kit (VisPRO™) and Pro-Q Diamond stain (Pro-Q, from Invitrogen) were used for developing total proteins or phosphoproteins.

 

 

 

 

Figure 2. Optimize suitable chromatographic condition with PhosPRO™ Phosphoprotein Purification Kit.

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Order Information :

Cat. No.	Product Name	Description
PP05-2C	PhosPRO™ Phosphoprotein  Purification Kit	Pre-packed column package 2 pre-packed column + 2ml Lysis buffer + 250ml System buffer + 100ml Elution buffer + 100ml stringent buffer stock
PP05-6C	PhosPRO™ Phosphoprotein Purification Kit	Pre-packed column packag 6 pre-packed column + 2ml Lysis buffer + 250ml System buffer + 100ml Elution buffer + 100ml stringent buffer stock
PP05-5E	PhosPRO™ Phosphoprotein Purification Kit	Evaluation package 3ml resin + 2ml Lysis buffer + 250ml System buffer + 100ml Elution buffer + 100ml stringent buffer stock