SDS-PAGE Protein Staining for Only 5 Minutes

VisPRO™ 5 Minutes Protein Stain Kit utilizes imidazole and zinc ions precipitate in the gel and reveal the protein position in only 5 minutes. The detection sensitivity can be reached to 1ng, competitive with silver stain and SYPRO ruby. VisPRO™ stains the gel rather than the protein sample and will not interact with the protein sample. The stained target protein can be eluted directly and apply downstream experiments, such as Western blotting, LCMSMS analysis. The staining solution is also highly stable, non-biohazard to operator and environment.

Special Characteristic

• Speed : quick staining procedure
• Sensitivity : detect protein level to 1ng
• Compatibility : applicable with downstream procedure 

Product Detail :

Figure 1. Positive staining vs. Reverse staining

Figure 2. Comparison the staining time by using VisPRO™ 5 minutes Protein Stain Kit and other staining.

Figure 3. Comparison of staining sensitivity. 

The commercial protein markers (Amersham, GE healthcare) were used for evaluation. Numbers shown above the gels indicate the actual amount of bovine serum albumin protein (indicated by arrow).

Figure 4. Compatibility of the VisPRO™ 5 minutes Protein Stain Kit with other applications.

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