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12035-069 MEP HyperCel Mixed-Mode 層析樹脂 5mL

12035-069

MEP HyperCel Mixed-Mode 層析樹脂 5mL

MEP HyperCel Mixed-Mode Chromatography Sorbent 5mL

廠牌:Pall Corp.
供應商:瑞柏生物科技股份有限公司
聯絡電話:02-28118200
定價:與我們聯絡

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快速詢價

3314

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MEP HyperCel Mixed-Mode 層析樹脂 5mL-行動條碼QR Code

 

Pall

Chromatography Products-Resins,Kits and Prepacked Columns

層析產品系列

依純化方式的不同,Pall的層析類產品可分為五大類:

1.依分子體積分餾與純化(Size Exclusion)-Ultrogel AcA & Trisacryl GF

用途:依分子體積分餾與純化;分子量的判定;去鹽。

特色:使用簡單,不會使蛋白質變性而影響其活性。

規格:

服服Particle Size Capacity Exclusion Limit Fracrionation Range
Ultrogel AcA 22 60-140μm n.a. 3,000 KD 100-1,200 KD
Ultrogel AcA 34 750 KD 20-350 KD
Ultrogel AcA 44 200 KD 10-130 KD
Ultrogel AcA 54 90 KD 5-70 KD
Ultrogel AcA 202 Desalting capacity 45% gel volume 22 KD 1-15 KD
Trisacryl GF05 註1 Desalting capacity 33% gel volume 3 KD 200-2,500 D
Trisacryl GF2000 n.a. 20,000 KD 10-15,000 KD
註1:GF05與GF2000均分為M grade 與LS grade,設計為實驗室使用的M grade濾珠大小差距較小(40-80μm);而設計供產程應用的LS grade(80-160μm)適合於壓力下以高流速進行分餾(最大可承受3bar的壓力)。

 

產品編號 內容 包裝
Ultrogel AcA
23015-025 Ultrogel AcA 34 100 mL
23015-019 Ultrogel AcA 34 1000 mL
23022-024 Ultrogel AcA 44 100 mL
23022-015 Ultrogel AcA 44 1000 mL
23019-023 Ultrogel AcA 54 100 mL
23019-011 Ultrogel AcA 54 1000 mL
24892-022 Ultrogel AcA 202 100 mL
24892-010 Ultrogel AcA 202 1000 mL
Trisacryl GF
25914-060 Trisacryl GF05 M 100 mL
25914-037 Trisacryl GF05 M 1000 mL
26065-045 Trisacryl GF2000 LS 100 mL
26065-011 Trisacryl GF2000 LS 1000 mL
 

2.以所帶電荷的親和力進行分離(Ion Exchange)-Q, S, DEAE & CM Ceramic HyperD Resins 陰離子交換 (陶瓷珠本身帶正電);Q (強) & DEAE (弱),陽離子交換 (陶瓷珠本身帶負電);S (強) & CM (弱)。

用途:蛋白質、多月太 (polypeptide)或質體的濃縮與純化。

特色:本類產品均由Pall專利陶瓷珠製成,不僅較傳統resin耐壓軸流速↑),且binding capacity亦高。

規格:

Product Particle Size Capacity Pressure Stability
Q Ceramic HyperD 20 20μm(avg) BSA:>85mg/ml 200bar
S Ceramic HyperD 20 Lysozyme:>85mg/ml
Q Ceramic HyperD F 50μm(avg) BSA:>85mg/ml 70bar
S Ceramic HyperD F Lysozyme:>85mg/ml
DEAE Ceramic HyperD F BSA:>85mg/ml
CM Ceramic HyperD F IgG:>60mg/ml
 

產品編號 內容 包裝
Bulk Resin
20040-051 Q Ceramic HyperD 20 5 mL
20040-044 Q Ceramic HyperD 20 25 mL
20040-036 Q Ceramic HyperD 20 100 mL
20040-028 Q Ceramic HyperD 20 500 mL
20040-010 Q Ceramic HyperD 20 1000 mL
20038-055 S Ceramic HyperD 20 5 mL
20038-048 S Ceramic HyperD 20 25 mL
20038-030 S Ceramic HyperD 20 100 mL
20038-022 S Ceramic HyperD 20 500 mL
20038-014 S Ceramic HyperD 20 1000 mL
20066-098 Q Ceramic HyperD F 5 mL
20066-031 Q Ceramic HyperD F 25 mL
20066-023 Q Ceramic HyperD F 100 mL
20066-015 Q Ceramic HyperD F 1000 mL
20062-089 S Ceramic HyperD F 5 mL
20062-030 S Ceramic HyperD F 25 mL
20062-022 S Ceramic HyperD F 100 mL
20062-014 S Ceramic HyperD F 1000 mL
20067-070 DEAE Ceramic HyperD F 5 mL
20067-039 DEAE Ceramic HyperD F 25 mL
20067-021 DEAE Ceramic HyperD F 100 mL
20067-013 DEAE Ceramic HyperD F 1000 mL
20050-084 CM Ceramic HyperD F 5 mL
20050-035 CM Ceramic HyperD F 25 mL
20050-027 CM Ceramic HyperD F 100 mL
20050-019 CM Ceramic HyperD F 1000 mL
Acrosep Columns
20050-C001 CM Ceramic HyperD F 1 mL
20062-C001 S Ceramic HyperD F 1 mL
20066-C001 Q Ceramic HyperD F 1 mL
20067-C001 DEAE Ceramic HyperD F 1 mL
IEXVP-C001 IEX Variety Pack 1 mL
Devices
5047 Acroprep 96 with Mustang Q, 350 μL 10/pkg
5062 Acroprep 96 with Mustang Q, 1 mL 5/pkg
5048 Acroprep 96 with Mustang S, 350 μL 10/pkg
5063 Acroprep 96 with Mustang S, 1 mL 5/pkg
MSTG25Q6 Acrodisc Mustang Q Chromatography Unit 10/pkg
MSTG25S6 Acrodisc Mustang S Chromatography Unit 10/pkg

3.依物質對 resin 上特定配體 (ligand) 的親和力進行純化 (Affinity)

  Product Ligand Capacity Application
RESIN Blue Trisacryl M Cibacron Blue F3GA chromophore HSA:10-15mg/ml
BSA:5-7mg/ml
Albumin depletion, purification of enzymes and proteins such as kinasws, interferons and some coagulation factors
Protein A Ceramic HyperD F Recomophore Human IgG:>30mg/ml IgG purification/depletion
Heparin HyperD M Porcine Heparin Human ATIII:>25mg/ml Purification of coagulation factors, lipoproteins, growth hormones, growth factors, nucleic acid binding enzymes
IMAC HyperCel" Metal Ions, such as CU++,Zn++, Co++, Ni++, GA+++, or FE+++ Metal ion capacity:40-70μmol Cu++/ml,Ionic capacity:90-140μeq/ml Purification of HIS-tagged proteins, antibodies and prefrationation of comples protein mixtures
Lysine HyperD Immobilized L-lysine n.a. Purification of glycoproteins and other biological molecules that bind to Lysine
 

產品編號 內容 包裝
Bulk Resin
25896-C001 Blue Trisacryl M , Column 1 mL
25896-051 Blue Trisacryl M 5 mL
25896-045 Blue Trisacryl M 25 mL
25896-010 Blue Trisacryl M 100 mL
25896-028 Blue Trisacryl M 1000 mL
20078-C001 Protein A Ceramic HyperD F , Column 1 mL
20078-036 Protein A Ceramic HyperD F 5 mL
20078-028 Protein A Ceramic HyperD F 25 mL
20078-010 Protein A Ceramic HyperD F 100 mL
20078-044 Protein A Ceramic HyperD F 1000 mL
20029-062 Heparin HyperD M 5 mL
20029-039 Heparin HyperD M 25 mL
20029-021 Heparin HyperD M 100 mL
20029-013 Heparin HyperD M 1000 mL
20093-C001 IMAC HyperCel , Column 1 mL
20093-069 IMAC HyperCel 5 mL
20093-010 IMAC HyperCel 25 mL
20093-028 IMAC HyperCel 100 mL
20059-058 Lysine HyperD 5 mL
20059-036 Lysine HyperD 25 mL
20059-028 Lysine HyperD 100 mL
20059-010 Lysine HyperD 1000 mL
Kits
5300-AFFMPS Enchant Multi-protein Affinity Separation Kit 24 purifications

4.利用兩種以上的特性進行分離與純化(Mixed Mode)

SDR HyperD MEP HyperCel
 

Product Mwthods Capacity Application
MEP HyperCel Hydrophobic Ineraction Electrostatic Repulsion Human IgG:>20mg/ml Purification/depletion of polyclonal and monoclonal antibodies from most species
SDR HyperD Hydrophilicity, Hydrophilicity & Size Exclusion (exclusion limit:10kDa) Triton X-100:60-80mg/ml Solvent-Detergent Removal while recovering NATIVE protein
HEA HyperCel Hydrophobic Interaction Electrostatic Repulsion BSA:40-60mg/ml (Recovery>90%) Unique industry-scalable sorbents designed foe protein capture and impurity removal in a abiopharmaceutical environment. Provides different selectivites not accessible with traditional ion exchange or HIC.
PPA HyperCel
 

產品編號 內容 包裝
Bulk Resin
12035-C001 MEP HyperCel , Column 1 mL
12035-069 MEP HyperCel 5 mL
12035-010 MEP HyperCel 25 mL
12035-028 MEP HyperCel 100 mL
12035-036 MEP HyperCel 1000 mL
20033-C001 SDR HyperD , Column 1 mL
20033-065 SDR HyperD 5 mL
20033-031 SDR HyperD 25 mL
20033-023 SDR HyperD 100 mL
20250-C001 HEA HyperCel , Column 1 mL
20250-012 HEA HyperCel 5 mL
20250-026 HEA HyperCel 25 mL
20250-033 HEA HyperCel 100 mL
20250-041 HEA HyperCel 1000 mL
20260-C001 PPA HyperCel , Column 1 mL
20260-015 PPA HyperCel 5 mL
20260-025 PPA HyperCel 25 mL
20260-030 PPA HyperCel 100 mL
20260-040 PPA HyperCel 1000 mL

5.Hu Ultrogel

用途:蛋白質、胜月太與核酸的分離與純化,尤其是鹼性蛋白質(cytochrome c, lysozyme)與磷酸化蛋白質。

特色:為Hydroxyapatile的微晶體鑲於agarose bead中。

Product Particle Size Capacity

HA Ultrogel

60-180μm

Cytochrome C:>7mg/ml
BSA:<7mg/ml

 

產品編號 內容 包裝
Bulk Resin
24775-075 HA UltroGel 5 mL
24775-082 HA UltroGel 25 mL
24775-025 HA UltroGel 100 mL
24775-041 HA UltroGel 1000 mL

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