20093-069 IMAC His-tagged 蛋白質純化專用層析樹脂 5mL
20093-069
IMAC His-tagged 蛋白質純化專用層析樹脂 5mL
IMAC HyperCel Affinity Chromatography Resin 5mL
廠牌：Pall Corp.
供應商：瑞柏生物科技股份有限公司
聯絡電話：02-28118200
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Pall
Chromatography Products-Resins，Kits and Prepacked Columns
層析產品系列
依純化方式的不同，Pall的層析類產品可分為五大類：
1.依分子體積分餾與純化(Size Exclusion)-Ultrogel AcA & Trisacryl GF
用途：依分子體積分餾與純化；分子量的判定；去鹽。
特色：使用簡單，不會使蛋白質變性而影響其活性。
規格：
| 服服 | Particle Size | Capacity | Exclusion Limit | Fracrionation Range |
| Ultrogel AcA 22 | 60-140μm | n.a. | 3,000 KD | 100-1,200 KD |
| Ultrogel AcA 34 | 750 KD | 20-350 KD | ||
| Ultrogel AcA 44 | 200 KD | 10-130 KD | ||
| Ultrogel AcA 54 | 90 KD | 5-70 KD | ||
| Ultrogel AcA 202 | Desalting capacity 45% gel volume | 22 KD | 1-15 KD | |
| Trisacryl GF05 | 註1 | Desalting capacity 33% gel volume | 3 KD | 200-2,500 D |
| Trisacryl GF2000 | n.a. | 20,000 KD | 10-15,000 KD | |
| 註1：GF05與GF2000均分為M grade 與LS grade，設計為實驗室使用的M grade濾珠大小差距較小(40-80μm)；而設計供產程應用的LS grade(80-160μm)適合於壓力下以高流速進行分餾(最大可承受3bar的壓力)。 | ||||
| 產品編號 | 內容 | 包裝 |
| Ultrogel AcA | ||
| 23015-025 | Ultrogel AcA 34 | 100 mL |
| 23015-019 | Ultrogel AcA 34 | 1000 mL |
| 23022-024 | Ultrogel AcA 44 | 100 mL |
| 23022-015 | Ultrogel AcA 44 | 1000 mL |
| 23019-023 | Ultrogel AcA 54 | 100 mL |
| 23019-011 | Ultrogel AcA 54 | 1000 mL |
| 24892-022 | Ultrogel AcA 202 | 100 mL |
| 24892-010 | Ultrogel AcA 202 | 1000 mL |
| Trisacryl GF | ||
| 25914-060 | Trisacryl GF05 M | 100 mL |
| 25914-037 | Trisacryl GF05 M | 1000 mL |
| 26065-045 | Trisacryl GF2000 LS | 100 mL |
| 26065-011 | Trisacryl GF2000 LS | 1000 mL |
2.以所帶電荷的親和力進行分離(Ion Exchange)-Q, S, DEAE & CM Ceramic HyperD Resins 陰離子交換 (陶瓷珠本身帶正電)；Q (強) & DEAE (弱)，陽離子交換 (陶瓷珠本身帶負電)；S (強) & CM (弱)。
用途：蛋白質、多月太 (polypeptide)或質體的濃縮與純化。
特色：本類產品均由Pall專利陶瓷珠製成，不僅較傳統resin耐壓軸流速↑)，且binding capacity亦高。
規格：
| Product | Particle Size | Capacity | Pressure Stability |  |
| Q Ceramic HyperD 20 | 20μm(avg) | BSA：>85mg/ml | 200bar | |
| S Ceramic HyperD 20 | Lysozyme：>85mg/ml | |||
| Q Ceramic HyperD F | 50μm(avg) | BSA：>85mg/ml | 70bar | |
| S Ceramic HyperD F | Lysozyme：>85mg/ml | |||
| DEAE Ceramic HyperD F | BSA：>85mg/ml | |||
| CM Ceramic HyperD F | IgG：>60mg/ml |
| 產品編號 | 內容 | 包裝 |
| Bulk Resin | ||
| 20040-051 | Q Ceramic HyperD 20 | 5 mL |
| 20040-044 | Q Ceramic HyperD 20 | 25 mL |
| 20040-036 | Q Ceramic HyperD 20 | 100 mL |
| 20040-028 | Q Ceramic HyperD 20 | 500 mL |
| 20040-010 | Q Ceramic HyperD 20 | 1000 mL |
| 20038-055 | S Ceramic HyperD 20 | 5 mL |
| 20038-048 | S Ceramic HyperD 20 | 25 mL |
| 20038-030 | S Ceramic HyperD 20 | 100 mL |
| 20038-022 | S Ceramic HyperD 20 | 500 mL |
| 20038-014 | S Ceramic HyperD 20 | 1000 mL |
| 20066-098 | Q Ceramic HyperD F | 5 mL |
| 20066-031 | Q Ceramic HyperD F | 25 mL |
| 20066-023 | Q Ceramic HyperD F | 100 mL |
| 20066-015 | Q Ceramic HyperD F | 1000 mL |
| 20062-089 | S Ceramic HyperD F | 5 mL |
| 20062-030 | S Ceramic HyperD F | 25 mL |
| 20062-022 | S Ceramic HyperD F | 100 mL |
| 20062-014 | S Ceramic HyperD F | 1000 mL |
| 20067-070 | DEAE Ceramic HyperD F | 5 mL |
| 20067-039 | DEAE Ceramic HyperD F | 25 mL |
| 20067-021 | DEAE Ceramic HyperD F | 100 mL |
| 20067-013 | DEAE Ceramic HyperD F | 1000 mL |
| 20050-084 | CM Ceramic HyperD F | 5 mL |
| 20050-035 | CM Ceramic HyperD F | 25 mL |
| 20050-027 | CM Ceramic HyperD F | 100 mL |
| 20050-019 | CM Ceramic HyperD F | 1000 mL |
| Acrosep Columns | ||
| 20050-C001 | CM Ceramic HyperD F | 1 mL |
| 20062-C001 | S Ceramic HyperD F | 1 mL |
| 20066-C001 | Q Ceramic HyperD F | 1 mL |
| 20067-C001 | DEAE Ceramic HyperD F | 1 mL |
| IEXVP-C001 | IEX Variety Pack | 1 mL |
| Devices | ||
| 5047 | Acroprep 96 with Mustang Q, 350 μL | 10/pkg |
| 5062 | Acroprep 96 with Mustang Q, 1 mL | 5/pkg |
| 5048 | Acroprep 96 with Mustang S, 350 μL | 10/pkg |
| 5063 | Acroprep 96 with Mustang S, 1 mL | 5/pkg |
| MSTG25Q6 | Acrodisc Mustang Q Chromatography Unit | 10/pkg |
| MSTG25S6 | Acrodisc Mustang S Chromatography Unit | 10/pkg |
3.依物質對 resin 上特定配體 (ligand) 的親和力進行純化 (Affinity)
| Product | Ligand | Capacity | Application | |
| RESIN | Blue Trisacryl M | Cibacron Blue F3GA chromophore | HSA：10-15mg/ml BSA：5-7mg/ml |
Albumin depletion, purification of enzymes and proteins such as kinasws, interferons and some coagulation factors |
| Protein A Ceramic HyperD F | Recomophore | Human IgG：>30mg/ml | IgG purification/depletion | |
| Heparin HyperD M | Porcine Heparin | Human ATIII：>25mg/ml | Purification of coagulation factors, lipoproteins, growth hormones, growth factors, nucleic acid binding enzymes | |
| IMAC HyperCel" | Metal Ions, such as CU++,Zn++, Co++, Ni++, GA+++, or FE+++ | Metal ion capacity：40-70μmol Cu++/ml，Ionic capacity：90-140μeq/ml | Purification of HIS-tagged proteins, antibodies and prefrationation of comples protein mixtures | |
| Lysine HyperD | Immobilized L-lysine | n.a. | Purification of glycoproteins and other biological molecules that bind to Lysine |
| 產品編號 | 內容 | 包裝 |
| Bulk Resin | ||
| 25896-C001 | Blue Trisacryl M , Column | 1 mL |
| 25896-051 | Blue Trisacryl M | 5 mL |
| 25896-045 | Blue Trisacryl M | 25 mL |
| 25896-010 | Blue Trisacryl M | 100 mL |
| 25896-028 | Blue Trisacryl M | 1000 mL |
| 20078-C001 | Protein A Ceramic HyperD F , Column | 1 mL |
| 20078-036 | Protein A Ceramic HyperD F | 5 mL |
| 20078-028 | Protein A Ceramic HyperD F | 25 mL |
| 20078-010 | Protein A Ceramic HyperD F | 100 mL |
| 20078-044 | Protein A Ceramic HyperD F | 1000 mL |
| 20029-062 | Heparin HyperD M | 5 mL |
| 20029-039 | Heparin HyperD M | 25 mL |
| 20029-021 | Heparin HyperD M | 100 mL |
| 20029-013 | Heparin HyperD M | 1000 mL |
| 20093-C001 | IMAC HyperCel , Column | 1 mL |
| 20093-069 | IMAC HyperCel | 5 mL |
| 20093-010 | IMAC HyperCel | 25 mL |
| 20093-028 | IMAC HyperCel | 100 mL |
| 20059-058 | Lysine HyperD | 5 mL |
| 20059-036 | Lysine HyperD | 25 mL |
| 20059-028 | Lysine HyperD | 100 mL |
| 20059-010 | Lysine HyperD | 1000 mL |
| Kits | ||
| 5300-AFFMPS | Enchant Multi-protein Affinity Separation Kit | 24 purifications |
4.利用兩種以上的特性進行分離與純化(Mixed Mode)
| SDR HyperD | MEP HyperCel |
 |
 |
| Product | Mwthods | Capacity | Application |
| MEP HyperCel | Hydrophobic Ineraction Electrostatic Repulsion | Human IgG：>20mg/ml | Purification/depletion of polyclonal and monoclonal antibodies from most species |
| SDR HyperD | Hydrophilicity, Hydrophilicity & Size Exclusion (exclusion limit：10kDa) | Triton X-100：60-80mg/ml | Solvent-Detergent Removal while recovering NATIVE protein |
| HEA HyperCel | Hydrophobic Interaction Electrostatic Repulsion | BSA：40-60mg/ml (Recovery>90%) | Unique industry-scalable sorbents designed foe protein capture and impurity removal in a abiopharmaceutical environment. Provides different selectivites not accessible with traditional ion exchange or HIC. |
| PPA HyperCel |
| 產品編號 | 內容 | 包裝 |
| Bulk Resin | ||
| 12035-C001 | MEP HyperCel , Column | 1 mL |
| 12035-069 | MEP HyperCel | 5 mL |
| 12035-010 | MEP HyperCel | 25 mL |
| 12035-028 | MEP HyperCel | 100 mL |
| 12035-036 | MEP HyperCel | 1000 mL |
| 20033-C001 | SDR HyperD , Column | 1 mL |
| 20033-065 | SDR HyperD | 5 mL |
| 20033-031 | SDR HyperD | 25 mL |
| 20033-023 | SDR HyperD | 100 mL |
| 20250-C001 | HEA HyperCel , Column | 1 mL |
| 20250-012 | HEA HyperCel | 5 mL |
| 20250-026 | HEA HyperCel | 25 mL |
| 20250-033 | HEA HyperCel | 100 mL |
| 20250-041 | HEA HyperCel | 1000 mL |
| 20260-C001 | PPA HyperCel , Column | 1 mL |
| 20260-015 | PPA HyperCel | 5 mL |
| 20260-025 | PPA HyperCel | 25 mL |
| 20260-030 | PPA HyperCel | 100 mL |
| 20260-040 | PPA HyperCel | 1000 mL |
5.Hu Ultrogel
用途：蛋白質、胜月太與核酸的分離與純化，尤其是鹼性蛋白質(cytochrome c, lysozyme)與磷酸化蛋白質。
特色：為Hydroxyapatile的微晶體鑲於agarose bead中。
| Product | Particle Size | Capacity | |
|
HA Ultrogel |
60-180μm |
Cytochrome C：>7mg/ml | |
 |
 | ||
| 產品編號 | 內容 | 包裝 |
| Bulk Resin | ||
| 24775-075 | HA UltroGel | 5 mL |
| 24775-082 | HA UltroGel | 25 mL |
| 24775-025 | HA UltroGel | 100 mL |
| 24775-041 | HA UltroGel | 1000 mL |
產品關鍵字：
