產品說明
- Principle : mini spin column (silica matrix)
- Sample size :
- Up to 200 μl whole blood, serum, plasma, body fluid
- Up to 5 x 106 cultured cells.
- Operation time : 30 minutes
- Binding capacity : up to 60 μg total DNA / column
- Expected yield : 4 ~ 8 μg / prep
- Column applicability : centrifugation and vaccum
- Minimum elution volume : 50 μl
Important Notes :
- Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
- Add 1.1 ml sterile ddH2O to Proteinase K tube to make a 10 mg/ml stock solution.
- Make sure that Proteinase K has been completely dissolved. Stor e the stock solution at 4 ℃ .
- Add ethanol (96- 100 %) to GW Buffer and Wash Buffer when first open.
- Prepare dry baths or water baths before the operation. One to 60℃ for step 2. Lysis
套組內容
APBGK 050/ APBGK 100/ APBGK 200
- BG Buffer 15ml/ 30ml /60 ml
- GW Buffer (concentrate) 22 ml/44ml/ 88ml
- Wash Buffer (concentrate) 10 ml/ 20ml/ 40m
- Elation Buffer 15ml/ 30ml/ 60ml
- Proteinase K 1 mg/ 11 mg x2/ 11mg x4
- BG Mini Column 50 pcs/ 100pcs/ 200pcs
- pcsCollection Tube. 100 pcs/200pcs/400pcs
- Elition.pTube. 50pcs/ 100 pcs/ 200 pcs
- User Manual x1