GTPO01 TOPfast PCR Cloning Kit
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TOPfast PCR Cloning Kit is suitable for cloning Taq polymerase amplified PCR products (with A-tail overhangs) at room temperature in 5 min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient ligation of DNA fragments with A-tail overhangs to the vector without using a regular ligase.
產品資訊
《快速簡單》
- TOPO cloning 系統
- 不需使用Ligase反應，僅需5分鐘即可完成 ligation反應。
- 使用Ampicillin、藍白篩選。《好用、高效率》
- Insert 長度至少可達3 kb
《好用、高效率》
- 載體帶有單一EcoR I切位, 容易轉殖 (subclone)。
Figure 1. Colony PCR results from colonies carrying different sized inserts.
PCR products of 300 bp (50 ng/µl)、1 kb (50 ng/µl) 、2 kb (100 ng/µl) and 3 kb (150 ng/µl) were ligated to TOPfast PCR Cloning Vectors, and colony PCR was performed using M13 primers.
For successful clones, the PCR result should be ~202 bp plus the insert size.
M : Gen-KB DNA Ladder
TOPfast Vector Information
產品內容物
| 1 vial of 20 μl | TOPfast Vector (10 ng/μl) |
| 1 vial of 20 μl | 6X TOPfastTM Buffer |
| 1 vial of 5 μl | Control insert DNA (1 kb) |
| 21 vials of 100 μl | DH5α Competent Cell |
| 1 vials 5 μl | Control Plasmid |
| 1 vial of 400 μl | M13 Control primer mix |
| 1 vial of 7.5 ml | SOC medium |
| 1 vial of 1 ml | Sterile water |
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