The most sensitive ECL substrate for Western blotting analysis

LumiFlash™ Infinity Chemiluminescent Substrate, HRP is an extremely sensitive enhanced chemiluminescent substrate for femtogram-level by detection of immobilized proteins (Western blotting). In Western blotting or ELISA application, LumiFlash™ Infinity Chemiluminescent Substrate, HRP provides high signal and low background, which allows detection of target protein on PVDF or nitrocellulose. This feature benefits the researchers with excellent low background result and without signal burn effect at the same time.

Special Characteristic

• High sensitivity – detect femtogram of protein target on PVDF or nitrocellulose membrane
• Antibody saving – use very low antibody concentration on the WB and ELISA application; dilute primary antibody 5000 to 20,000-fold and secondary antibody 100,000 to 400,000-fold (from 1mg/mL stock)
• Low background – provide insignificant background and avoid non-specific signals on the WB result.   

Product Detail :

Figure 1. The comparison of WB application for LumiFlash Infinity and Luminata Forte.

Hela cell lysate with 1/2 serial dilution from 20 µg was separated by 12.5% SDS-PAGE and probed by anti AMPKa1. All results were exposed to X-ray film for 5 minutes.

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Reference:

1. jin Jang, Yeo, et al. "The Effect of Pharmacopuncture at GV20 on Dementia in a Focal Cerebral Ischemia Mice Model." The Acupuncture 34.3 (2017): 1-11.

2. Kim, Seung Cheol, Boyun Choi, and Youngjoo Kwon. "Thiol-reducing agents prevent sulforaphane-induced growth inhibition in ovarian cancer cells." Food & nutrition research 61.1 (2017): 1368321.

3. Kwon, Youngjoo. "Food-derived polyphenols inhibit the growth of ovarian cancer cells irrespective of their ability to induce antioxidant responses." Heliyon 4.8 (2018): e00753.

4. Yang, Yi-Feng, and Yao-Jen Liang. "Adenine decreases hypertrophic effects through interleukin-18 receptor." Chinese Journal of Physiology 62.4 (2019): 139

5. Chang, Chao-Chien, et al. "Lipopolysaccharide promoted proliferation and adipogenesis of preadipocytes through JAK/STAT and AMPK-regulated cPLA2 expression." International journal of medical sciences 16.1 (2019): 167.

6. Hsu, Pei-Sung, et al. "Participation of NADPH Oxidase-Related Reactive Oxygen Species in Leptin-Promoted Pulmonary Inflammation: Regulation of cPLA2α and COX-2 Expression." International journal of molecular sciences 20.5 (2019): 1078.