HybriMore Hybridoma Culture Supplement (舊名: HybriMore Hybridoma Cloning Factor) 是一款 chemical-defined 不含動物來源成分之融合瘤細胞專用培養添加劑。本添加劑可提高融合瘤細胞之fusion後存活率，進而提升後續建立抗體生產細胞株之成功率。

Growth promoting supplement especially for hybridoma cells

HybriMore™, Hybridoma cloning factor is a special supplement which is adding in the medium when culturing hybridoma cells. It can substantially provide necessary growth factor during cell culture and therefore successfully increase the cloning efficiency and raise the survival rate of hybridoma cell. No negative effect to hybridoma cell with defined chemical component and defined concentration.

Special Characteristic

• Increase cloning efficiency and cell survival rate
• Culturing hybridoma cells with better antibody secretion and production
• Growth promoting supplement with defined chemical component and concentration

Product Detail :

Figure 1. Comparison of the cloning efficiency of the newly fused hybridoma cells.

The newly PEG fused hybridoma cells were plated into a 96-well plate containing FCS media with HybriMore™ (green bars), FCS media with feeder layer (blue bars), or regular FCS media (white bars). Hybridoma cells were subject to HAT selection 14 days after the cell fusion. The numbers of viable hybridoma colonies were visually counted under a microscope. Two mouse myeloma fusion partners, NS-1 and SP2/0, were evaluated by four independent fusion experiments with freshly prepared mouse spleens.

Figure 2. Comparison of successful rate of monoclonizing hybridoma cells.

Eight clones of hybridoma cells from NS-1 or SP2/0 fusion partners were monoclonized in the media containing FCS media with HybriMore™ (green bars), FCS media with feeder layer (blue bars), or regular FCS media (white bars). The numbers of viable hybridoma colonies in a well were visually counted under a microscope.

Figure 3. Comparison of the titers of secreting Ab.

A clone of hybridoma cells (anti human transferrin, L3B5) was cultured in the media containing FCS media with HybriMore (green bars), FCS media with feeder layer (blue bars), or regular FCS media (white bars) for seven days. The supernatants were harvest and examined by the titer of secreting Ab by ELSIA assay

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