教學 YouTube :  https://youtu.be/BxWJFxaNFMY

* 實驗判讀總是需要拿底片與 membrane 模糊對照？
* 需要操作麻煩的冷光影像重疊？
* western 沒有看到結果，到底是冷光試劑失效還是其他地方出錯？
* 在暗房裡面，一不小心就搞混了實驗結果，沒有一眼可見的記號，真是麻煩！

目前研究人員進行 Western blot 時，廣泛使用高靈敏度的冷光系統來偵測實驗結果，然而，採用冷光系統，作為分子量參考指標之 standard markers，因其蛋白質不與實驗抗體結合，而無法呈現冷光，因此在 X-ray film 上不會呈現markers資訊，研究者因而損失重要資訊—分子量對照。

LuminolPen 是一枝在墨水中添加了冷光試劑與顏料的筆，當您進行 western blot 實驗時，只要在 transfer 之後，blocking 之前，以LuminolPen 在membrane 按照 pre-stained markers 的位置描上去，再加註分子量或其他實驗資訊，當完成後續實驗步驟後，在 X-ray film 或冷光影像中就會呈現
您所註記上去的資訊。

Product Detail :

Figure 1. Labeling molecular weight and note on the membrane with LuminolPen™.

 (A) and (C) are general Western blot chemiluminescence detection image; (B) and (D) are the image using LuminolPen™ to indicate the pre-stained marker.

Figure 2. Distinguish target signal and non-specific binding signal with LuminolPen™

30 μg cell lysate SP-1 and detect with anti-ADNP (mouse, 1:2,000). Secondary antibody : anti-mouse IgG-HRP (1:15,000). Membrane : Hyond™ P. Detection : Hyperfilm™ ECL. Signal exposed for 30 seconds and detected by X-ray. The molecular weight note by LuminolPen™ reveal the correct target signal (ADNP 124KDa) on X-ray film.

Figure 3. Evaluate ECL substrate efficacy by LuminolPen™.

30 μg cell lysate HepaG2 and detect with anti-AMP-active protein kinase (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP (1:10,000). Membrane : Hyond™ P. Detection : Hyperfilm™ ECL. Signal exposed for 30 seconds and detected by X-ray. Signal result present by normal ECL substrate is stronger than using expired ECL substrate. LuminolPen™ noted marker can be an indicator to check the result or adjust different experiment to similar experimental condition.

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